Qualitative and Quantitative Standardization of Andrographis paniculata by TLC Technique and UV Method
Abstract
Author(s): Javed Ahamad, Muath Sh Mohammed Ameen, Esra T Anwer, Subasini Uthirapathy, and Omji Porwal
Background: Andrographis paniculata Wall. commonly known as Kalmegh and widely used for the treatment of the liver complications in Indian sub-continent. It is important ingredient of several traditional herbal formulations. Materials and Methods: Hence we planned to developed quick qualitative and quantitative standardization method for its quality control by using TLC and UV-visible spectroscopic methods for its better therapeutic efficacy. Results: TLC and UV-visible spectroscopic method has been developed for identification and quantification of andrographolide in A. paniculata herb samples. The developed TLC resulted in well resolved spots of different constituents of herb along with andrographolide standard. The developed UV-visible spectroscopic method was used for the estimation of andrographolide in A. paniculata herb samples. Conclusion: These quick standardization methods are used as analytical tool for the routine analysis of A. paniculata herb samples for achieving better therapeutic effects.
